PfCor-N produces parallel actin bundles observed by TIRF microscopy. 2 μM Mg-ATP-actin with 1 μM Oregon Green Mg-ATP-actin (a) alone (b) + 0.1 μM PfCor-N. Arrowheads indicate growing ends of filaments. Scale bars 5 μm. c Pixel intensity quantification of a and b across 45 frames represented as measure of filament bundling. Actin alone pixel intensity mode = 701 arbitrary units (au) (red line). 100 nM PfCor-N pixel intensity mode = 1,189 au (red line). d Actin assembled in presence of 100 nM Fimbrin. Top panel antiparallel bundle, bottom panel parallel bundle. Arrowheads indicate growing ends of the filaments. Scale bar 15 μm.