Figure 6

PfCoronin is expressed in schizonts/merozoites and is located at the periphery of mature merozoites. a RT-PCR of P. falciparum cDNA for PfCoronin and PfACTI genes across asexual life-cycle post invasion (pi). gDNA positive control (with introns). Negative control—no reverse transcriptase. b Western blots across asexual life-cycle post invasion (pi), probed with anti-PfCoronin, anti-PfACT1 (anti-Act239-253 (rabbit), [10]), anti-PfAldolase [40] and anti-PfAMA1 [60]. c Western blot WT 3D7 P. falciparum versus PfCoroninHA, probed with anti-HA. d Reciprocal western blots of immunoprecipitations from 3D7 PfCoroninHA schizont lysates with anti-PfCoronin bait, probed with anti-HA (left panel) or anti-HA bait, probed with anti-PfCoronin (right panel). Control lanes = WT 3D7 P. falciparum. Arrow indicates PfCoroninHA. Asterisks heavy and light chain cross-reactivity. e IFA of schizonts probed with anti-HA (Coronin, red), DAPI (nucleus, blue), anti-GAP45 (IMC, green) (middle panel) [40] or anti-PfACTI (IMC/cytosol green) (bottom panel). Scale bar 2 μm.