Fig. 6

Giemsa staining of Plasmodium vivax parasites cultured in McCoy’s 5A medium and MMM. The invasion assay was performed by cultured purified schizonts with RP in 2 different culture media, McCoy’s 5A medium and MMM medium. At 4 h of cultured in both media, parasites successfully reinvaded the new reticulocytes and formed ring stage parasites. When the maturation was continued to observe at 48 h, parasites cultured in MMM medium developed to mature schizont stage which contained more than 5 nuclei and brown pigment. The infected erythrocytes were enlarged, very pale staining and contained pink dots (Schüffner’s dots) scattered through out the cytoplasm. While the parasites cultured in McCoy’s 5A medium has not reached the schizont stage.