Efficacy of artemether–lumefantrine therapy for the treatment of uncomplicated Plasmodium falciparum malaria in Southwestern Ethiopia

Table 1 Specific primers for msp1 and msp2 primer sets

Locus	Allele	Primer		Sequence (5′ to 3′)
Locus	Allele	Forward	Reverse	Sequence (5′ to 3′)
msp1	N/A	✔		CTAGAAGCTTTAGAAGATGCAGTATTG
	N/A		✔	CTTAAATAGTATTCTAATTCAAGTGGATCA
	K1	✔		AAATGAAGAAGAAATTACTACAAAAGGTGC
	K1		✔	GCTTGCATCAGCTGGAGGGCTTGCACCAGA
	MAD20	✔		AAATGAAGGAACAAGTGGAACAGCTGTTAC
	MAD20		✔	ATCTGAAGGATTTGTACGTCTTGAATTACC
	RO33	✔		TAAAGGATGGAGCAAATACTCAAGTTGTTG
	RO33		✔	CATCTGAAGGATTTGCAGCACCTGGAGATC
msp2	N/A	✔		ATGAAGGTAATTAAAACATTGTCTATTATA
			✔	ATATGGCAAAAGATAAAACAAGTGTTGCTG
	FC27	✔		GCAAATGAAGGTTCTAATACTAATAG
			✔	GCTTTGGGTCCTTCTTCAGTTGATTC
	3D7/IC	✔		AGAAGTATGGCAGAAAGTAAKCCTYCTACT
			✔	GATTGTAATTCGGGGGATTCAGTTTGTTCG

All the primer sequences were adopted from Worldwide Antimalarial Resistance Network, WWARN (2005).

ISSN: 1475-2875