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Fig. 3 | Malaria Journal

Fig. 3

From: Inhibition by stabilization: targeting the Plasmodium falciparum aldolase–TRAP complex

Fig. 3

PfAldolase catalytic activity and thermal stability in the presence of compounds. a Normalized bar graph representing the change in ∆Vmax relative to the control in the presence of each of the 60 ordered compounds. All assays were carried out in the presence of TRAP as used for the reference control. A negative ∆Vmax indicates further inhibition of glycolysis in the presence of the compound compared to PfAldolase with TRAP only. A positive ∆Vmax indicates displacement of TRAP by the compound resulting in an increased catalytic activity. Compounds inhibiting invasion in an in vitro culture assay are numbered in the plot (see Fig. 5). b Thermal stability assay derivative curves of four controls of PfAldolase with PfTRAP. The TM is indicted by the minima of the derivative at approximately 65 °C. c Average of a triplicate thermal stability assay in the presence of small molecules with and without PfTRAP peptide. The green bar represents the melting temperature of the control without compound. The change in thermal stability (∆TM) in the presence of PfTRAP per compound is depicted in the last graph. The dotted line represents the cutoff of a shift greater than 1.5 °C.

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