Inhibition assay of Rabbit and Plasmodium aldolase with compound 24. Rabbit aldolase was utilized as a surrogate for human aldolase due to the sequence identity. Different concentrations of compound 24 were tested in the presence and absence of the TRAP-peptide. No apparent inhibition is observed in either rabbit or Plasmodium aldolase alone, while a strong inhibition is observed when TRAP is present. Assays were performed in triplicate under standard conditions.