Fig. 2

Detection of alleles in artificial mixtures of plasmid DNA by applying different criteria for rare allele identification. The frequency of detection of multiple alleles was calculated considering all 11 of the artificial mixtures assayed for each marker. The bars represent the total proportion of infections identified by each marker. Two criteria for minor allele detection were considered: a cut-off value of one-third (colored in black only) or one-fourth (the entire bar, including both black and grey bars), of the height of the predominant peak. The increase in the rate of detection of alleles with one-fourth criterion is highlighted in grey