Origin | Assay | Diagnostic accuracy | No. of samples | Type of sample | PCR technique used for speciation | Pf | Pv | Pm | Pk | Po spp | Mixed infections | Negatives | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Papua New Guinea | 42 | Filter paper | Multiplex PCR | 12 | 9 | 0 | 0 | 0 | 5 (Pf/Pv) | 16 | |||
HtLAMP-Pg | Sensitivity |
77 % (20/26) 95 % CI 56–91 | |||||||||||
Specificity |
81 % (13/16) 95 % CI 54–96 | ||||||||||||
HtLAMP-Pf | Sensitivity |
76 % (13/17) 95 % CI 50–93 | |||||||||||
Specificity |
100 % (25/25) 95 % CI 86–100 | ||||||||||||
Sabah, Malaysia | 145 | Filter paper | Multiplex PCR and nested PCR (Pk) | 17 | 64 | 7 | 56 | 0 | 1 (Pv/Pk) | 0 | |||
HtLAMP-Pg | Sensitivity |
100 % (145/145) 95 % CI 97–100 | |||||||||||
HtLAMP-Pf | Sensitivity |
100 % (17/17) 95 % CI 80–100 | |||||||||||
Specificity |
95 % (123/128) 95 % CI 90–98 | ||||||||||||
The Gambia | 97 | Whole blood | Nested PCR | 94 | NA | 1 | NA | 0 | 0 | 2 | |||
HtLAMP-Pg | Sensitivity |
100 % (95/95) 95 % CI 96–100 | |||||||||||
Specificity |
100 % (2/2) 95 % CI 16–100 | ||||||||||||
HtLAMP-Pf | Sensitivity |
100 % (94/94) 95 % CI 96–100 | |||||||||||
Specificity |
100 % (3/3) 95 % CI 29–100 | ||||||||||||
TOTAL | 284 | 124 | 73 | 8 | 56 | 0 | 6 | 18 | |||||
HtLAMP-Pg | Sensitivity |
98 % (260/266) 95 % CI 95–99 | |||||||||||
Specificity |
83 % (15/18), 95 % CI 59–96 | ||||||||||||
PPV | 99 %, 95 % CI 97–100 | ||||||||||||
NPV | 71 %, 95 % CI 48–89 | ||||||||||||
HtLAMP-Pf | Sensitivity |
97 % (124/128) 95 % CI 92–99 | |||||||||||
Specificity |
96 % (151/157) 95 % CI 92–99 | ||||||||||||
PPV | 95 %, 95 % CI 90–98 | ||||||||||||
NPV | 97 %, 95 % CI 94–99 |