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Table 3 Sensitivity and specificity of HtLAMP-Pg/Pf for the detection of P. falciparum

From: A simple, high-throughput, colourimetric, field applicable loop-mediated isothermal amplification (HtLAMP) assay for malaria elimination

Origin Assay Diagnostic accuracy   No. of samples Type of sample PCR technique used for speciation Pf Pv Pm Pk Po spp Mixed infections Negatives
Papua New Guinea     42 Filter paper Multiplex PCR 12 9 0 0 0 5 (Pf/Pv) 16
HtLAMP-Pg Sensitivity 77 % (20/26)
95 % CI 56–91
          
  Specificity 81 % (13/16)
95 % CI 54–96
          
HtLAMP-Pf Sensitivity 76 % (13/17)
95 % CI 50–93
          
  Specificity 100 % (25/25)
95 % CI 86–100
          
Sabah, Malaysia     145 Filter paper Multiplex PCR and nested PCR (Pk) 17 64 7 56 0 1 (Pv/Pk) 0
HtLAMP-Pg Sensitivity 100 % (145/145)
95 % CI 97–100
          
HtLAMP-Pf Sensitivity 100 % (17/17)
95 % CI 80–100
          
  Specificity 95 % (123/128)
95 % CI 90–98
          
The Gambia     97 Whole blood Nested PCR 94 NA 1 NA 0 0 2
HtLAMP-Pg Sensitivity 100 % (95/95)
95 % CI 96–100
          
  Specificity 100 % (2/2)
95 % CI 16–100
          
HtLAMP-Pf Sensitivity 100 % (94/94)
95 % CI 96–100
          
  Specificity 100 % (3/3)
95 % CI 29–100
          
TOTAL     284    124 73 8 56 0 6 18
HtLAMP-Pg Sensitivity 98 % (260/266)
95 % CI 95–99
          
  Specificity 83 % (15/18),
95 % CI 59–96
          
  PPV 99 %, 95 % CI 97–100           
  NPV 71 %, 95 % CI 48–89           
HtLAMP-Pf Sensitivity 97 % (124/128)
95 % CI 92–99
          
  Specificity 96 % (151/157)
95 % CI 92–99
          
  PPV 95 %, 95 % CI 90–98           
  NPV 97 %, 95 % CI 94–99           
  1. This table summarizes the features of each of the 3 sample sets, from Papua New Guinea, Sabah Malaysia and The Gambia, and a composite sensitivity and specificity of HtLAMP-Pg/Pf for the detection of P. falciparum