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Table 3 Sensitivity, specificity, and false negative rate of each primer set in faecal samples

From: Non-invasive surveillance for Plasmodium in reservoir macaque species

  Number of faecal samples PCR + in paired blood sample 1 PCR run 2 PCR runs
Sensitivitya Specificity FNRb Sensitivity Specificity FNR
ssrRNA 32 30 96.7 % (83.3–99.4 %) 100 % 0.03 NA NA NA
cytb-a 64 34 29.4 % (16.8–46.2 %) 100 % 0.71 29.4 % (19.9–41.2 %) 100 % 0.71
cytb-b 64 34 29.4 % (16.8–46.2 %) 100 % 0.71 23.5 % (14.9–35.0 %) 100 % 0.76
cytb-c 64 34 41.2 % (26.4–57.8 %) 100 % 0.59 41.2 % (30.2–53.0 %) 100 % 0.59
  1. aThe sensitivity is the percentage of infected macaques that are positive by PCR from faecal samples. 95% confidence intervals (CI) are given in parenthesis
  2. bFNR is the false negative rate, or the number of false negatives divided by the sum of false negatives and true positives