Skip to main content

Table 4 Species specific sensitivity, specificity, positive predictive value and negative predictive value of microscopy compared with HVUSqPCR parasite detection

From: The epidemiology of subclinical malaria infections in South-East Asia: findings from cross-sectional surveys in Thailand–Myanmar border areas, Cambodia, and Vietnam

  qPCR pos micro pos qPCR pos micro neg qPCR neg micro neg qPCR neg micro pos Total Sensitivity (95 % CI) Specificity (95 % CI) Positive predictive value (95 % CI) Negative predictive value (95 % CI)
Pf
 All sites 62 144 4616 5 4827 30 % (24–37 %) 100 % (99–100 %) 93 % (83–98 %) 97 % (96–97 %)
 Cam 0 36 1410 1 1447 0 % (0–10 %) 100 % (99–100 %) 0 % (0–10 %) 98 % (97–98 %)
 TMBA 37 61 1418 1 1517 38 % (28–48 %) 100 % (99–100 %) 98 % (86–100 %) 96 % (95–97 %)
 Viet 25 47 1788 3 1863 35 % (24–47 %) 100 % (99–100 %) 89 % (72–98 %) 97 % (97–98 %)
Pv
 All sites 141 262 4411 13 4827 35 % (30–40 %) 100 % (99–100 %) 92 % (86–95 %) 94 % (94–95 %)
 Cam 7 45 1395 0 1447 14 % (6–26 %) 100 % (99–100 %) 100 % (59–100 %) 97 % (96–98 %)
 TMBA 99 149 1264 5 1517 40 % (34–46 %) 100 % (99–100 %) 95 % (89–98 % 89 % (89–91 %)
 Viet 35 68 1752 8 1863 34 % (25–44 %) 100 % (99–100 %) 81 % (67–92 %) 96 % (95–97 %)
P. spp
 All sites 13 391 3864 1 4269 3 % (2–5 %) 100 % (99–100 %) 93 % (66–100 %) 91 % (90–92 %)
 Cam 0 145 1218 0 1363 0 % (0–0.3 %) 100 % (97–100 %) NA 89 % (88–91 %)
 TMBA 6 175 1009 0 1190 3 % (1–7 %) 100 % (99–100 %) 100 % (54–100 %) 85 % (83–87 %)
 Viet 7 71 1637 1 1716 9 % (4–18 %) 100 % (99–100 %) 88 % (47–100 %) 96 % (95–97 %)
  1. HVUSqPCR, high volume ultra-sensitive real time polymerase chain reaction; Pf, Plasmodium falciparum or Plasmodium falciparum mixed infection; Pv, Plasmodium vivax; P. spp., Plasmodium species not identified by HVUSqPCR compared against any Plasmodium infection detected by RDT; Cam, Cambodia; TMBA, Thailand–Myanmar border areas; qPCR pos, positive (species specific); qPCR neg, negative; NA, not applicable