Table 1 Primary antibodies used in the study
Antibodies | Type | Source | Dilution | Measuring site |
|---|---|---|---|---|
CD3 | Mouse monoclonal | DakoCytomation® | 1:100 | All T-lymphocyte |
CD8 | Mouse monoclonal | DakoCytomation® | 1:100 | Cytotoxic T-lymphocyte |
CD68 | Mouse monoclonal | DakoCytomation® | 1:200 | Macrophage |
Neutrophil elastase | Mouse monoclonal | DakoCytomation® | 1:200 | Neutrophil |
IL-33 | Rabbit polyclonal | Milipore® | 1:100 | Bronchial epithelium |
γ-ENaC | Rabbit polyclonal | Milipore® | 1:100 | Bronchial epithelium Vascular smooth muscle cell |
AQP-1 | Rabbit polyclonal | Milipore® | 1:100 | Alveolar capillary |
AQP-5 | Rabbit polyclonal | Milipore® | 1:50 | Alveolar epithelium |
Pan cytokeratin | Rabbit polyclonal | Milipore® | 1:400 | Bronchial epithelium |