Impact of chrysosplenetin on the pharmacokinetics and anti-malarial efficacy of artemisinin against Plasmodium berghei as well as in vitro CYP450 enzymatic activities in rat liver microsome

Table 1 Method validation for quantification of ART in rat plasma

QC (ng/mL)	Precision and accuracy (%)		Stability (RE%)				Recovery (%)	Matrix effect (n = 5) Mean (%)/RSD%
	Inter-day RSD/RE	Intra-day RSD/RE	Pre-T		Post-T	Freeze–thaw for three times
	Inter-day RSD/RE	Intra-day RSD/RE	RT 6 h	RT 24 h	RT 24 h	Freeze–thaw for three times
0.5	8.51/1.24	6.57/1.24	−4.00	−2.00	2.00	2.00	57.30	98.0/1.70
10	1.41/6.75	6.67/−1.90	3.20	−0.80	−8.20	6.80	73.30	88.0/2.70
160	2.97/7.81	6.99/0.67	−6.25	−3.00	−4.38	4.38	68.90	66.0/3.10

ISSN: 1475-2875