Skip to main content

Table 1 Method validation for quantification of ART in rat plasma

From: Impact of chrysosplenetin on the pharmacokinetics and anti-malarial efficacy of artemisinin against Plasmodium berghei as well as in vitro CYP450 enzymatic activities in rat liver microsome

QC (ng/mL) Precision and accuracy (%) Stability (RE%) Recovery (%) Matrix effect (n = 5)
Mean (%)/RSD%
Inter-day
RSD/RE
Intra-day
RSD/RE
Pre-T Post-T Freeze–thaw for three times
RT
6 h
RT
24 h
RT
24 h
0.5 8.51/1.24 6.57/1.24 −4.00 −2.00 2.00 2.00 57.30 98.0/1.70
10 1.41/6.75 6.67/−1.90 3.20 −0.80 −8.20 6.80 73.30 88.0/2.70
160 2.97/7.81 6.99/0.67 −6.25 −3.00 −4.38 4.38 68.90 66.0/3.10
  1. RT room temperature, Pre-T Pre-treatment, Post-T post-treatment