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Table 3 Method validation for quantification of PN, CA, OPZ, DM, CLZ, and MDZ in inactive RLM

From: Impact of chrysosplenetin on the pharmacokinetics and anti-malarial efficacy of artemisinin against Plasmodium berghei as well as in vitro CYP450 enzymatic activities in rat liver microsome

QC (μM) PN QC (μM) CA QC (μM) OPZ
Precision and accuracy (%) Average recovery (%, n = 5) Precision and accuracy (%) Average recovery (%, n = 5) Precision and accuracy (%) Average recovery (%, n = 5)
Intra- Inter- Absolute Method Intra- Inter- Absolute Method Intra- Inter- Absolute Method
a
 3.0 1.19 8.87 106.72 (1.77) 97.17 (1.19) 3.4 0.46 6.64 112.49 (1.12) 113.17 (0.46) 2.8 2.80 2.67 93.45 (7.79) 77.96 (6.02)
 28.0 1.44 3.08 99.12 (3.05) 93.84 (3.30) 34.2 4.15 4.09 97.86 (2.92) 72.37 (4.15) 14.5 0.61 0.78 99.98 (3.70) 94.30 (1.52)
 89.0 7.25 2.32 97.24 (1.22) 87.12 (1.42) 100.0 6.12 4.33 101.32 (6.68) 94.97 (6.12) 58.0 1.67 1.64 97.01 (0.32) 100.16 (0.65)
QC (μM) CLZ QC (μM) MDZ QC (μM) DM
Precision and accuracy (%) Average recovery (%, n = 5) Precision and accuracy (%) Average recovery (%, n = 5) Precision and accuracy (%) Average recovery (%, n = 5)
Intra- Inter- Absolute Method Intra- Inter- Absolute Method Intra- Inter- Absolute Method
b
 6.0 1.26 3.83 94.74 (3.00) 94.48 (0.92) 3.0 3.87 5.29 98.51 (0.9) 100.50 (0.37) 2.7 11.92 10.50 81.50 (12.15) 104.70 (11.92)
 29.5 1.31 4.90 97.14 (1.05) 95.31 (0.58) 15.0 1.93 3.36 100.07 (9.05) 107.18 (1.06) 13.5 5.56 8.56 96.75 (6.46) 98.33 (5.56)
 118.0 2.96 1.97 102.60 (3.26) 97.12 (1.90) 49.0 0.17 2.53 93.42 (4.47) 98.79 (4.43) 43.2 4.43 2.37 99.96 (3.03) 94.45 (2.26)
  1. The numbers in the brackets represented RSD%