Table 3 Method validation for quantification of PN, CA, OPZ, DM, CLZ, and MDZ in inactive RLM
QC (μM) | PN | QC (μM) | CA | QC (μM) | OPZ | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Precision and accuracy (%) | Average recovery (%, n = 5) | Precision and accuracy (%) | Average recovery (%, n = 5) | Precision and accuracy (%) | Average recovery (%, n = 5) | |||||||||
Intra- | Inter- | Absolute | Method | Intra- | Inter- | Absolute | Method | Intra- | Inter- | Absolute | Method | |||
a | ||||||||||||||
 3.0 | 1.19 | 8.87 | 106.72 (1.77) | 97.17 (1.19) | 3.4 | 0.46 | 6.64 | 112.49 (1.12) | 113.17 (0.46) | 2.8 | 2.80 | 2.67 | 93.45 (7.79) | 77.96 (6.02) |
 28.0 | 1.44 | 3.08 | 99.12 (3.05) | 93.84 (3.30) | 34.2 | 4.15 | 4.09 | 97.86 (2.92) | 72.37 (4.15) | 14.5 | 0.61 | 0.78 | 99.98 (3.70) | 94.30 (1.52) |
 89.0 | 7.25 | 2.32 | 97.24 (1.22) | 87.12 (1.42) | 100.0 | 6.12 | 4.33 | 101.32 (6.68) | 94.97 (6.12) | 58.0 | 1.67 | 1.64 | 97.01 (0.32) | 100.16 (0.65) |
QC (μM) | CLZ | QC (μM) | MDZ | QC (μM) | DM | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Precision and accuracy (%) | Average recovery (%, n = 5) | Precision and accuracy (%) | Average recovery (%, n = 5) | Precision and accuracy (%) | Average recovery (%, n = 5) | |||||||||
Intra- | Inter- | Absolute | Method | Intra- | Inter- | Absolute | Method | Intra- | Inter- | Absolute | Method | |||
b | ||||||||||||||
 6.0 | 1.26 | 3.83 | 94.74 (3.00) | 94.48 (0.92) | 3.0 | 3.87 | 5.29 | 98.51 (0.9) | 100.50 (0.37) | 2.7 | 11.92 | 10.50 | 81.50 (12.15) | 104.70 (11.92) |
 29.5 | 1.31 | 4.90 | 97.14 (1.05) | 95.31 (0.58) | 15.0 | 1.93 | 3.36 | 100.07 (9.05) | 107.18 (1.06) | 13.5 | 5.56 | 8.56 | 96.75 (6.46) | 98.33 (5.56) |
 118.0 | 2.96 | 1.97 | 102.60 (3.26) | 97.12 (1.90) | 49.0 | 0.17 | 2.53 | 93.42 (4.47) | 98.79 (4.43) | 43.2 | 4.43 | 2.37 | 99.96 (3.03) | 94.45 (2.26) |