Quantification of Plasmodium ex vivo drug susceptibility by flow cytometry

Malaria Journal

Table 3 Difference in IC₅₀ estimates (nM) derived by light microscopy (LM) and flow cytometry (FC)

Anti-malarial	P. falciparum				P. vivax
Anti-malarial	n	Mean [95 % CI] difference in IC₅₀ LM-FC (nM)	Median [Range] difference in IC₅₀ LM-FC (nM)	Δ IC₅₀ LM-FC p value^a	n	Mean [95 % CI] difference in IC₅₀ LM-FC (nM)	Median [Range] difference in IC₅₀ LM-FC (nM)	Δ IC₅₀ LM-FC p value^a
Chloroquine	54	−3.8 [−10.7 to 3.2]	−5.1 [−60.9–59.4]	0.124	21	−4.5 [−16.0 to 7.1]	−5.4 [−46.1–60.2]	0.725
Piperaquine	54	6.0 [3.8 to 8.2]	6.1 [−18.7–21.3]	<0.001	21	0.7 [−4.6 to 6.0]	−4.5 [−15.2–27.5]	0.425
Mefloquine	39	1.3 [−0.6 to 3.1]	0.1 [−5.7–18.6]	0.969	14	1.2 [−3.6 to 6.0]	2.8 [−13.9–15.8]	0.931
Amodiaquine	24	4.0 [1.9 to 6.0]	2.8 [−2.7–15.1]	0.002	13	4.7 [−4.3 to 13.7]	0.4 [−11.8–40.2]	0.864
Artesunate	38	−0.4 [−0.7 to −0.01]	−0.25 [−3.6–2.9]	0.077	17	−0.12 [−0.9 to 0.7]	−0.45 [−2.2–5.0]	0.102

CI confidence interval
^a p-values obtained from paired t- test on log-transformed IC₅₀ derived by LM and FC

ISSN: 1475-2875