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Table 4 IC50 values derived by different staining methods in P. falciparum laboratory strains and clinical Plasmodium isolates

From: Quantification of Plasmodium ex vivo drug susceptibility by flow cytometry

Anti-malarial Mean IC50 (nM) in P. falciparum laboratory linesa Clinical Plasmodium isolates
FC27 (CQ sensitive) K1 (CQ resistant) P. falciparum (n = 5)
Median IC50 (nM, range)
P. vivax (n = 2)
IC50 estimates (nM)
Singleb Doublec LMd Singleb Doublec LMd Singleb Doublec LM Singlee Doublec LMd
CQ 25.1 23.7 20.7 171.0 167.0 159.4 85.0 (52.8–165.0) 97.3 (52.9–179.0) 108 (65.6–115.0) 92.2; 132.0 88.5; 150.0 102.0; 113.0
PIP 32.8 32.5 34.7 63.0 63.9 89.7 35.0 (19.9–45.0) 38.6 (12.6–57.1) 47.5 (27.6–68.4) 79.1; 153.0 85.6; 155.0 91.8; 115.0
MFQ 46.8 40.9 49.9 16.6 14.5 9.4 10.2 (3.5–19.3) 10.2 (2.2–23.3) 9.4 (3.6–15.5) 23.0; 43.0 30.3; 42.4 17.7; 22.6
AS 4.1 4.3 2.6 8.7 7.8 6.0 2.2 (1.3–3.1) 2.2 (1.9–3.7) 1.9 (1.2–2.6) 2.6; 6.3 2.8; 6.7 3.1; 7.8
  1. CQ chloroquine, PIP piperaquine, MFQ mefloquine, AS artesunate
  2. aMean IC50s (derived from 2 independent experiments)
  3. bAssay quantification by flow cytometry (FC) using single staining with hydroethidine (HE) for P. falciparum
  4. cAssay quantification by FC using double staining with HE and SG for both species [27, 29]
  5. d LM light microscopy
  6. eAssay quantification by flow cytometry (FC) using single staining with SYBR Green I (SG) for P. vivax