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Table 4 IC50 values derived by different staining methods in P. falciparum laboratory strains and clinical Plasmodium isolates

From: Quantification of Plasmodium ex vivo drug susceptibility by flow cytometry

Anti-malarial

Mean IC50 (nM) in P. falciparum laboratory linesa

Clinical Plasmodium isolates

FC27 (CQ sensitive)

K1 (CQ resistant)

P. falciparum (n = 5)

Median IC50 (nM, range)

P. vivax (n = 2)

IC50 estimates (nM)

Singleb

Doublec

LMd

Singleb

Doublec

LMd

Singleb

Doublec

LM

Singlee

Doublec

LMd

CQ

25.1

23.7

20.7

171.0

167.0

159.4

85.0 (52.8–165.0)

97.3 (52.9–179.0)

108 (65.6–115.0)

92.2; 132.0

88.5; 150.0

102.0; 113.0

PIP

32.8

32.5

34.7

63.0

63.9

89.7

35.0 (19.9–45.0)

38.6 (12.6–57.1)

47.5 (27.6–68.4)

79.1; 153.0

85.6; 155.0

91.8; 115.0

MFQ

46.8

40.9

49.9

16.6

14.5

9.4

10.2 (3.5–19.3)

10.2 (2.2–23.3)

9.4 (3.6–15.5)

23.0; 43.0

30.3; 42.4

17.7; 22.6

AS

4.1

4.3

2.6

8.7

7.8

6.0

2.2 (1.3–3.1)

2.2 (1.9–3.7)

1.9 (1.2–2.6)

2.6; 6.3

2.8; 6.7

3.1; 7.8

  1. CQ chloroquine, PIP piperaquine, MFQ mefloquine, AS artesunate
  2. aMean IC50s (derived from 2 independent experiments)
  3. bAssay quantification by flow cytometry (FC) using single staining with hydroethidine (HE) for P. falciparum
  4. cAssay quantification by FC using double staining with HE and SG for both species [27, 29]
  5. d LM light microscopy
  6. eAssay quantification by flow cytometry (FC) using single staining with SYBR Green I (SG) for P. vivax