Table 1 Enzymes involved in sugar nucleotide pathways, GPI-anchor and C-, N- and O-glycan biosynthesis of P. falciparum

1

Hexokinase (HK)

EC 2.7.1.1

PF3D7_0624000

Pv, Pk, Pc, Pr, Pb, Py, Pch

2

Glucose-6-phosphate isomerase (G6PI)

EC 5.3.1.9

PF3D7_1436000

Pv, Pk, Pc, Pr, Pb, Py, Pch

3

Glucosamine-fructose-6-phosphate aminotransferase (GFPT)

EC 2.6.1.16

PF3D7_1025100

Pv, Pk, Pc, Pr, Pb, Py, Pch

4

Glucosamine-phosphate N- acetyltransferase (GNA)

EC 2.3.1.4

No gene identified

5

Phosphoacetylglucosamine mutase (PAGM)

EC 5.4.2.3

PF3D7_1130000

Pv, Pk, Pc, Pr, Pb, Py, Pch

6

UDP-N-acetylglucosamine pyrophosphorylase (UAP)

EC 2.7.7.23

PF3D7_1343600

Pv, Pk, Pc, Pr, Pb, Py, Pch

7

Mannose-6-phosphate isomerase (MPI)

EC 5.3.1.8

PF3D7_0801800

Pv, Pk, Pc, Pr, Pb, Py, Pch

8

Phosphomannomutase (PMM)

EC 5.4.2.8

PF3D7_1017400

Pv, Pk, Pc, Pr, Pb, Py, Pch

9

Mannose-1-phosphate guanyltransferase (MPG)

EC 2.7.7.13

PF3D7_1420900

Pv, Pk, Pc, Pr, Pb, Py, Pch

10

GDP-mannose 4,6-dehydratase (GMD)

EC 4.2.1.47

PF3D7_0813800

Pv, Pk, Pc, Pr, Pb, Py, Pch

11

GDP-L-fucose synthase (FS)

EC 1.1.1.271

PF3D7_1014000

Pv, Pk, Pc, Pr, Pb, Py, Pch

12

Phosphoglucomutase (PGM)

EC 5.4.2.2

PF3D7_1012500

Pv, Pk, Pc, Pr, Pb, Py, Pch

13

UTP-glucose-1-phosphate uridylyltransferase (UGP) or UDP-sugar pyrophosphorylase (USP)

EC 2.7.7.9 or EC 2.7.7.64

PF3D7_0517500

Pv, Pk, Pc, Pr, Pb, Py, Pch

14

Galactokinase (GK)

EC 2.7.1.6

No gene identified

15

UDP-glucose 4-epimerase (GALE)

EC 5.1.3.2

No gene identified

16

Dolichol-phosphate mannosyltransferase polypeptide 1 (DPM1)

EC 2.4.1.83

PF3D7_1141600

Pv, Pk, Pc, Pr, Pb, Py, Pch

Enzymes involved in GPI-Anchor biosynthesis

17

 phosphatidylinositol n- acetylglucosaminyltransferase (PIG-A)

EC 2.4.1.198

PF3D7_0618900.1 and PF3D7_0935300 and/or PF3D7_1032400 and/or PF3D7_1141400

Pv, Pk, Pc, Pr, Pb, Py, Pch

18

 N-acetylglucosaminyl phosphatidylinositol deacetylase (PIG-L)

EC 3.5.1.89

PF3D7_0624700 and/or PF3D7_0911000

Pv, Pk, Pc, Pr, Pb, Py, Pch

19

 Inositol acyltransferase (PIG-W)

EC 2.3

PF3D7_0615300

Pv, Pk, Pc, Pr, Pb, Py, Pch

20

 GPI mannosyltransferase I (PIG-M)

EC 2.4.1

PF3D7_1210900

Pv, Pk, Pc, Pr, Pb, Py, Pch

21

 GPI mannosyltransferase II (PIG-V)

EC 2.4.1

PF3D7_1247300

Pv, Pk, Pc, Pr, Pb, Py, Pch

22

 GPI mannosyltransferase III (PIG-B)

EC 2.4.1

PF3D7_1341600

Pv, Pk, Pc, Pr, Pb, Py, Pch

23

 GPI mannosyltransferase IV

EC 2.4.1

No gene identified

Enzymes involved in N-glycans biosynthesis

24

 UDP-N-Acetyl-glucosamine-1-P transferase (ALG7)

EC 2.7.8.15

PF3D7_0321200

Pv, Pk, Pc, Pr, Pb, Py, Pch

25

 UDP-N-Acetylglucosaminyltransferase subunit (ALG13)

EC 2.4.1.141

PF3D7_0806400

Pv, Pk, Pc, Pr, Pb, Py, Pch

26

 UDP-N-Acetylglucosaminyltransferase subunit (ALG14)

EC 2.4.1.141

PF3D7_0211600

Pv, Pk, Pr, Pb, Py, Pch

27

 Catalytic subunit of the oligosaccharyltransferase complex (STT3)

EC 2.4.99.18

PF3D7_1116600

Pv, Pk, Pc, Pr, Pb, Py, Pch

Enzymes involved in O-fucosylation

28

 GDP-fucose protein O-fucosyltransferase 2 (PoFUT2)

EC 2.4.1.221

PF3D7_0909200

Pv, Pk, Pc, Pr, Pb, Py, Pch

Enzymes involved in C-mannosylation

29

 C-mannosyltransferase

EC 2.4.1

PF3D7_0806200

Pv, Pk, Pc, Pr, Pb, Py, Pch