Antiproliferative effect of amitozyn on eukaryotic cells and culture of Plasmodium falciparum.
a IC50 of amitozyn for CS2, 3G8 and NF 54 strains of P. falciparum. Human RBC at 2 % haematocrit were infected with CS2, 3G8 and NF54 strains of P. falciparum. At 0.5 % parasitaemia infected RBC were treated with 0–125 μg/mL amitozyn for 96 h and then stained by Giemsa’s method. Parasitaemia was analysed by inverted light microscope as described in “Methods”. The data were statistically treated and plotted on the graphs using GrahPad software. IC50 values were found from the graph. b Comparison of IC50 of amitozyn in eukaryotic cell cultures and in the culture of P. falciparum (CS2, 3G8 and NF54 lines). Eukaryotic cells were exposed to 0–500 μg/mL amitozyn. At 72 h cells were fixed with 3.7 % formaldehyde and stained with 0.1 % crystal violet. Ratio of viable cells was analysed as described in “Methods”. The value of IC50 was estimated from cell viability plots and presents the average value of the three independent experiments