Fig. 6

Isobolographic analysis of combined effects of amitozyn with chloroquine, pyrimethamine and artemisinin in the culture of Plasmodium falciparum (3G8). Calculation of CI values of a amitozyn/chloroquine, b amitozyn/pyrimethamine and c amitozyn/artemisinin combinations at antiparasitic effects from 10 to 90 %. Human RBC at 2 % haematocrit were infected with 3G8 line of P. falciparum. At 0.5 % parasitaemia iRBC were treated with amitozyn (0–60 μg/mL), chloroquine (0–30 nM), pyrimethamine (0–20 nM), artemisinin (0–4 nM), and the combinations of amitozyn with chloroquine, pyrimethamine and artemisinin in the molar ratio 778:1, 778:1 and 11,679:1, respectively, for 96 h. CI was calculated as described in “Methods”