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Fig. 1 | Malaria Journal

Fig. 1

From: A comparison of Plasmodium falciparum circumsporozoite protein-based slot blot and ELISA immuno-assays for oocyst detection in mosquito homogenates

Fig. 1

ECL-SB integrated optical density values for mosquito homogenate bands exposed by X-ray. The capacity of the slot blot apparatus was 48 mosquito homogenates, including three to five negative controls for the calculation of CSP positivity. Each blot was developed separately, giving rise to the varied IOD values in the figure. Positivity determination was thus blot-specific, based on the mean IOD values of the negative controls obtained in each blot. As the total number of mosquitoes processed at each time point in groups 2 and 4 exceeded the capacity of a single blot (48 test mosquitoes plus controls), a small number of test mosquitoes from these groups were processed in separate blots (eight and five mosquitoes from each time point for groups 2 and 4, respectively), the results of which are shown in Additional file 1: Figure S1. For clarity, the primary figure shows blots containing the majority of mosquitoes from all groups and time points

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