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Table 1 Means, standard deviations, and seropositivity cutoff values for each antigen as determined by different immunoassays and statistical methods

From: Multiple comparisons analysis of serological data from an area of low Plasmodium falciparum transmission

Distribution, assay Antigen Malaria-naïve reference population Finite mixture model, first component
Mean (SD) Mean + 3SD Mean + 5SD Mean (SD) Mean + 3SD Mean + 5SD
Normal distribution
 ELISA (in OD) MSP1-42(D) 0.045 (0.027) 0.126 0.180 0.125 (0.069) 0.332 0.470
MSP1-42(F) 0.030 (0.019) 0.087 0.125 0.127 (0.068) 0.331 0.467
MSP1-19 0.052 (0.025) 0.127 0.177 0.157 (0.080) 0.397 0.557
AMA-1 0.056 (0.038) 0.170 0.246 0.14 (0.069) 0.347 0.485
 Multiplex (in MFI) MSP1-42(D) 10.7 (21.2) 74.3 116.7 18.3 (16.7) 68.4 101.8
MSP1-42(F) 16.4 (29.1) 103.7 161.9 21.7 (22.4) 88.9 133.7
MSP1-19 11.1 (10.7) 43.2 64.6 11.4 (10.5) 42.9 63.9
AMA-1 15.3 (19.0) 72.3 110.3 21.7 (23.5) 92.2 139.2
Lognormal distribution
 ELISA (in OD) MSP1-42(D) 0.045 (0.027) 0.126 0.180 0.085 (0.572) 1.801 2.945
MSP1-42(F) 0.030 (0.021) 0.093 0.135 −1.214 (1.242) 2.512 4.996
MSP1-19 0.052 (0.026) 0.130 0.182 0.377 (0.385) 1.532 2.302
AMA-1 0.056 (0.037) 0.167 0.241 0.292 (0.500) 1.792 2.792
 Multiplex (in MFI) MSP1-42(D) 10.1 (17.0) 61.1 95.1 2.5 (0.96) 5.4 7.3
MSP1-42(F) 15.3 (21.6) 80.1 123.3 2.5 (0.69) 4.6 6.0
MSP1-19 10.9 (9.8) 40.3 59.9 2.0 (0.84) 4.5 6.2
AMA-1 15.0 (16.6) 64.8 98.0 2.5 (0.91) 5.2 7.1