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Fig. 2 | Malaria Journal

Fig. 2

From: Identifying rapidly parasiticidal anti-malarial drugs using a simple and reliable in vitro parasite viability fast assay

Fig. 2

Optimization of erythrocyte labelling for fluorescence-activated cell sorting detection. Graphs represent the ratios of mean fluorescence intensities from positive cells with respect to the negative population. Error bars represent SEM for three replicates. a Optimization of the assay used different concentrations of CFDA-SE (20, 10 and 5 µM), were tested to stain erythrocytes using 1 or 2 % haematocrit. b Signal intensity by labelled erythrocytes remained robust for detection at least 7 days after staining (CFDA-SE 10 µM, 1 % haematocrit). c To test whether a reduction in incubation time could be achieved, different times (30 min, 1 and 2 h) were used for staining erythrocytes with 10 µM CFDA-SE at 1 % haematocrit

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