Fig. 1

The WD40-repeat protein PfWLP1 co-immunoprecipitates with PfCCp1 and is transcribed in the Plasmodium falciparum blood stages. a Silver-stained SDS-PAGE of co-immunoprecipitated proteins from lysates of non-activated (Gc) and activated (aGc) gametocytes using anti-PfCCp1 antisera as bait. Erythrocyte lysate (Ec) used as negative control. A protein running at ~100 kDa (asterisks) was identified by mass spectrometry as product of gene PF3D7_1443400, termed PfWLP1. b Schematic of PfWLP1. The five annotated WD40 motifs (yellow boxes) are represented. The underlined regions denote the recombinant proteins used for antisera generation. c Transcription of pfwlp1 in the blood stages. Diagnostic RT-PCR was used to amplify pfwlp1 transcript (272 bp) from ring stages (Ring), trophozoites (Troph), schizonts (Schiz), immature (imGc), mature (mGc) and activated gametocytes (aGC). Transcript analyses of pfama1 (180 bp) and pfccp2 (187 bp) were used to demonstrate purity of the asexual blood stage and gametocyte samples, respectively. Transcript analysis of pfaldolase (378 bp) was used for loading control. Data are representative of three independent experiments