Fig. 3

PfWLP1 is expressed in the sexual stages of Plasmodium falciparum. a Expression of PfWLP1 in non-activated and activated gametocytes. PfWLP1 was immunolabelled with anti-PfWLP1rp2 antisera (green); the sexual stages were visualized with anti-Pfs230 antisera or by Evans Blue (EB) counterstaining (red). The parasite nuclei were highlighted by Hoechst nuclear stain (in blue). b Co-localization of PfWLP1 with distinct cell structures of gametocytes. PfWLP1 was immunolabelled with anti-PfWLP1rp2 antisera (green). The plasma membrane-associated adhesion protein Pfs230 and the inner membrane complex component PfGAP45 were visualized using the respective rabbit antisera (red), nuclei were stained with Hoechst (blue). c Localization of PfWLP1 underneath the gametocyte plasma membrane. Image enlargement (right) of a stage IV gametocyte, labelled with antibodies against PfWLP1 (green) and Pfs230 (red), nuclei were stained with Hoechst (blue). IMC inner membrane complex, GPM gametocyte plasma membrane. Bar 5 µm (a, b), 1 μm (c). Data are representative of five independent experiments