Fig. 4

Expression of PfWLP1-HA in asexual blood stages and gametocytes. a Detection of PfWLP1-HA in parasite lysates. Western blot analysis of lysates from ring stages (Ring), trophozoites (Troph), schizonts (Schiz), immature gametocytes (imGc), mature gametocytes (mGc) and activated gametocytes (aGc) of line PfWLP1-HA, using rabbit anti-HA antibody, detected PfWLP1-HA migrating at the expected size of ~108 kDa. Lysates of WT asexual blood stages (WT ABs) and gametocytes (WT Gc) as well as of non-infected erythrocytes (Ec) was used as negative controls. Equal loading of lanes was confirmed by blotting with mouse antisera against Pf39 (~39 kDa). b PfWLP1 expression in the PfWLP1-HA asexual blood stages. PfWLP1-HA was immunolabelled with anti-HA antisera (green); the asexual blood stages were visualized by Evans Blue counterstaining (red). The parasite nuclei were highlighted by Hoechst nuclear stain (blue). c PfWLP1 expression in the PfWLP1-HA non-activated and activated gametocytes. PfWLP1-HA was immunolabelled with anti-HA antisera (green). The sexual stages were visualized with antisera against Pfs230 (red); nuclei were stained with Hoechst (blue). Bar 5 µm. Data are representative of two to five independent experiments