Fig. 4

RT-PCR identification of the ace-1 ^R mutation. Scatter plots of the fluorescence of ace-1 ^R mutation detection. Sequence SS the DNA samples identified as sensitive homozygous by the sequencing method, sequence RS identified as heterozygous, sequence RR identified as resistant homozygous, SS control the plasmid coding the ace-1 ^S sequence, RS control mixture of the plasmid coding the ace-1 ^S sequence and that coding the ace-1 ^R sequence, RR control the plasmid coding the ace-1 ^R sequence, negative control distilled water instead of template DNA