Detection of G119S ace-1 R mutation in field-collected Anopheles gambiae mosquitoes using allele-specific loop-mediated isothermal amplification (AS-LAMP) method

Table 1 Comparing the detection of the ace-1 ^R mutation using AS-LAMP and PCR-RFLP

Genotype	Number tested					Sensitivity (95 % CL)			Specificity (95 % CL)
Genotype	Sequencing	PCR-RFLP	RT-PCR	AS-LAMP	PCR-RFLP	RT-PCR	AS-LAMP	PCR-RFLP	RT-PCR	AS-LAMP
Ace1RR	1	1	0	1	1	0	1	1	1	1
					(0.21–1.00)	(0–0.79)	(0.21–1.00)	(0.96–1)	(0.96–1)	(0.96–1)
Ace1RS	9	9	12	8	1	0.75	0.89	1	1	1
					(0.79–1.00)	(0.47–0.91)	(0.56–0.98)	(0.96–1)	(0.96–1)	(0.96–1)
Ace1SS	94	94	92	95	0.99	0.98	1	0.90	1	0.90
					(0.94–0.99)	(0.93–0.99	(0.96–1)	(0.60–0.98)	(0.72–1)	(0.60–0.98)
Total	104	104	104	104	–		–	–		–

Genotypes and associated number of mosquitoes detected using PCR-RFLP, RT-PCR, and AS-LAMP methods, compared to sequencing results. Percent sensitivity and specificity of each method relative to the sequencing are in parentheses

ISSN: 1475-2875