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Fig. 3 | Malaria Journal

Fig. 3

From: Molecular characterization of misidentified Plasmodium ovale imported cases in Singapore

Fig. 3

Dimorphic nature of Plasmodium ovale curtisi and Plasmodium ovale wallikeri based on the partial sequences from three nuclear and two mitochondrial genes. a 18S ssrRNA, b Porbp2, c Pog3p, d cox1, e cytb genes. Each alignment includes sequences from the P. ovale cases imported in Singapore (highlighted in grey) and from published sequences. Numbering is based for the 18S ssrRNA gene on the asexually expressed (A type) gene [L48987], for the Porbp2 and Pog3p genes on the putative start-codons (ATG) identified by [23], for the cox1 gene on the homologous position of the P. falciparum cox1 gene [M76611], and for the cytb gene on the start-codon (ATG). For 18S ssrRNA gene the sets of sequences A1 and A2 contain: [L48986, L48987, AB182489, GQ231515, GQ183065, GQ183068] and [GU813972, JF894422, JF894425, JF894426] respectively; for Porbp2 gene the sets of sequences B1 and B2 contain: [GU813972, JF894422, JF894425, JF894426] and [GU813971, JF894427-JF894429] respectively; for cytb gene the sets of sequences E1 to E4 gene contain [HQ712053, GQ231518-GQ231520], [GU723535-GU723537, GU723539-GU723548], [HQ712052, AB354571, GQ231516-GQ231517] and [AB182496, AF069625, GU723514-GU723534], respectively. The Singapore P. o. wallikeri and Singapore P. o. curtisi sets represent for each gene the sequences obtained from [Po 2006, Po 2012-3 and Po-2014-2] and from [Po 2003, Po 2012-1, Po 2012-2, Po 2012-4, Po 2013-1, Po 2013-2, Po 2013-3 and Po-2014-1], respectively. Non-synonymous mutations are in boldface type, hyphens represent gaps, dots represent nucleic acid identity and blanks represent unavailable information

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