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Fig. 4 | Malaria Journal

Fig. 4

From: Molecular characterization of misidentified Plasmodium ovale imported cases in Singapore

Fig. 4

Dimorphic nature of Plasmodium ovale curitsi and Plasmodium ovale wallikeri based on the translated amino acid sequences from two additional nuclear genes. a Potra gene, and b dhfr-ts gene. Each alignment includes sequences from the imported P. ovale cases imported in Singapore (highlighted in grey) and from published sequences. Dimorphic sites are in boldface type. For Potra gene, the distinction of between P. o. wallikeri types 1 and 2 is based on a two amino acid variation located outside of the amplified fragment presented here [24], and the distinction between P. o. curtisi types 1 and 2 is based on the number of PISTIT repeat (n = 1 or 2) [23, 24]. For dhfr-ts gene, in addition to the dimorphisms between P. o. wallikeri and P. o. curtisi, the amino acid polymorphisms within each species are included to illustrate internal variations. In Potra gene for P. o. wallikeri the green and blue boxes represent the PINMAN repeats (n = 1–5) region and the PINAIT repeats (n = 2–4) region, respectively; for P. o. curtisi the orange box represents the PISTIT repeat (n = 1 or 2) region. In dhfr-ts the red box represents the AT repeats (n = 1 or 2) region. Numbering is based on the ORF identified by [24] for Potra, and on the putative start-codon (ATG) of dhfr-ts. Hyphens represent gaps and dots represent amino acid identity

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