Fig. 2

Device in operation. For clarity the weight which would rest over the location of the sample pad has been omitted and the edges of the sample and running buffer sliders have been outlined. a Sample slider at position 1, mixing RPA reagents and target and allowing amplification to occur (30 min). b After amplification, the sample slider pulled down to position 2, in contact with dry dilution pads to absorb RPA buffer (10 min). c The sample slider pulled down to position 3, in contact with wet dilution pads, for dilution with TBST (10 min). d Sample slider pulled down to position 4 into contact with lateral flow strip. The running buffer slider is pulled down to activate the strip (5 min)