Skip to main content

Table 1 Alkaloids identified in Aspidosperma parvifolium trunk bark alkaloid extract by UPLC-DAD-ESI–MS/MS and HPLC-ESI-MICROTOF-MS

From: Aspidosperma species as sources of anti-malarials: uleine is the major anti-malarial indole alkaloid from Aspidosperma parvifolium (Apocynaceae)

Compounds Peak number Rt (min) UPLC-ESI-MS UV λmax (nm) UPLC-DAD [M+H]+
UPLC-ESI-MS (m/z)
HRMS HPLC-ESI-
MICROTOF-MS (m/z)
UPLC-ESI-MS/MS fragments (m/z)
Spegazzinidine 1 2.06 317.13 373.43   373.11, 354.90, 341.10, 323.90, 311.42, 295.0, 279.10, 247.79, 231.91, 205.95, 183.71, 124.07, 108.86
1,13-Dihydro-13- hydroxyuleine 2 2.26 316.13 285.36 285.1949 285.11, 254.11, 238.10, 236.29, 210.16, 208.0, 206.01, 197.97, 195.15, 182.15, 168.15, 167.02, 155.96, 144.02, 130.02, 124.07, 121.89
Aspidocarpine 3 2.50 304.13 371.40 371.1975 371.22, 353.95, 339.21, 321.0, 270.22, 249.68, 245.90, 227.69, 220.13, 199.71, 173.75, 154.10, 152.08, 142.88, 107.97
Deacetylaspidospermine 4 2.60 317.13 313.38 313.1953 313.08, 251.89, 222.09, 206.08, 180.12, 168.03, 151.96, 140.05, 131.92
Pyrifolidine 5 2.62 317.13 385.45 385.14, 367.12, 353.07, 327.11, 309.09, 256.10, 231.97, 167.89, 121.07
1-Methyldeacetyl-aspidospermidine 6 2.88 310.13 327.37 327.2061 327.01, 309.16, 296.15, 283.14, 278.30, 252.21, 236.19, 197.97, 170.0, 168.24, 130.72, 129.21
Dasycarpidone 7 2.74 319.13 269.35 269.1628 238.16, 210.12, 209.04, 192.16, 182.11, 167.06, 130.01
Aspidospermidin-19-ol 8 3.25 302.13 299.40 299.1806 299.15, 252.02, 234.75, 233.75, 221.33, 208.10, 206.34, 196.07, 169.16, 159.39, 150.07, 138.03, 122.97, 109.17
N-Acetylaspidospermidine 9 3.27 303.13 341.35 341.1856 341.15, 322.96, 309.17, 304.58, 295.20, 294.07, 281.29, 266.8, 251.90, 236.22, 223.01, 183.73, 168.12, 121.86, 107.82
Des-N-methyluleine 10 3.35 304.13 253.34 253.1686 236.10, 221.10, 208.09, 207.16, 206.03, 194.09, 193.03, 182.03, 181.03, 167.02
Apparicine 11 3.39 303.13 265.36 265.1678 265.11, 250.02, 236.12, 222.16, 208.14, 207.20, 206.09, 194.12, 180.0, 158.03, 134.01, 108.17
Uleine 12 3.49 303.13 267.39 267.1845 236.10, 221.04, 208.09, 207.09, 206.16, 193.03, 182.09, 167.02
Epi-uleine 13 3.53 304.13 267.69 267.1853 236.16, 221.04, 208.09, 207.03, 206.03, 194.03, 193.03, 182.03, 181.03, 167.02
Aspidospermine 14 3.63 297.13 355.46 355.1993 355.14, 323.30, 313.08, 268.19, 256.22, 218.07, 173.81, 164.17, 124.01, 122.05
Dasycarpidol 15 3.88 304.13 283.40 283.1793 236.23, 221.04, 208.09, 207.09, 206.16, 193.09, 192.15, 181.50, 167.0
Dasycarpidan 16 5.51 303.13 281.30 - 281.05, 236.07, 222.08, 194.04
  1. (−) species not detected
  2. Rt retention time, UV ultraviolet, UPLC-DAD-ESI–MS ultra performance liquid chromatography photo diode array electron spray mass spectrometry, HRMS high resolution mass spectrometry, HPLC-ESI-MICROTOF-MS high performance liquid chromatography electro spray ionization time-of-flight mass spectrometry