Genetic diversity of Plasmodium falciparum parasite by microsatellite markers after scale-up of insecticide-treated bed nets in western Kenya

Table 2 Genetic diversity of parasites in (a) Asembo, Gem and Karemo areas, 2007 survey and, (b) Asembo 2001 and 2007 surveys

(a) Locus	Asembo		Gem		Karemo		p value
(a) Locus	Allele no. and (richness)	H _e (SE)	Allele no. and (richness)	H _e (SE)	Allele no. and (richness)	H _e (SE)	Asembo/Gem	Asembo/Karemo	Gem/Karemo
Polya	16 (15.94)	0.91 (0.0193)	17 (16.78)	0.89 (0.0279)	19 (18.85)	0.88 (0.0315)	0.620	0.469	0.814
Pfg377	5 (4.99)	0.47 (0.0726)	6 (5.74)	0.41 (0.0648)	5 (4.72)	0.32 (0.059)	0.472	0.073	0.316
PfPK2	10 (10)	0.85 (0.0243)	11 (10.44)	0.82 (0.0209)	12 (11.32)	0.84 (0.0221)	0.353	0.749	0.530
ADL	12 (12)	0.88 (0.0177)	14 (13.71)	0.90 (0.0114)	16 (15.28)	0.91 (0.0089)	0.205	0.075	0.550
EBP	8 (8)	0.82 (0.0562)	14 (13.4)	0.88 (0.0388)	11 (10.84)	0.83 (0.0232)	0.426	0.886	0.312
P195^a	7 (6.99)	0.74 (0.0249)	6 (5.83)	0.65 (0.0206)	6 (5.99)	0.74 (0.0209)	0.005	0.957	0.003
TAA60	8 (7.98)	0.81 (0.0249)	9 (8.42)	0.79 (0.0206)	7 (6.96)	0.79 (0.0209)	0.561	0.550	0.983
TAA109	9 (8.97)	0.79 (0.0249)	12 (11.28)	0.83 (0.0169)	14 (13.17)	0.83 (0.0239)	0.124	0.178	0.993
Overall		0.78 (0.0495)		0.77 (0.0060)		0.77 (0.0661)	0.813	0.859	0.972

(b) Locus	Asembo-2001		Asembo-2007		p value
(b) Locus	Allele no. and (richness)	H _e (SE)	Allele no. and (richness)	H _e (SE)	p value
Poly-α	17 (16.92)	0.92 (0.0243)	16 (15.94)	0.91 (0.0193)	0.653
Pfg377	3 (3.00)	0.57 (0.0984)	5 (4.96)	0.47 (0.0726)	0.489
PfPK2	12 (11.92)	0.83 (0.0497)	10 (10.00)	0.85 (0.0243)	0.752
ADL	14 (13.96)	0.89 (0.0191)	12 (12.00)	0.88 (0.0177)	0.623
EBP	6 (5.99)	0.82 (0.0193)	8 (8.00)	0.82 (0.0562)	0.976
P195	11 (10.91)	0.75 (0.0241)	7 (6.99)	0.74 (0.0249)	0.733
TAA60	17 (16.87)	0.85 (0.0322)	8 (7.98)	0.81 (0.0249)	0.344
TAA109	11 (10.94)	0.77 (0.0372)	9 (8.97)	0.79 (0.0249)	0.691
Overall		0.79 (0.0380)		0.78 (0.0495)	0.625

Asembo, Gem and Karemo denotes years after introduction of ITNs; 10, 9 and 3 years, respectively
Comparison of genetic diversity between areas and between years was based on the number of alleles, allele richness (between areas only), unbiasied heterozygosity (H _e) and standard error (SE) [31]. Standard error was calculated to generate a p value for statistical testing of differences in H _e
p value <0.05 are in italics
^aDenotes locus with significantly different H _e between areas

ISSN: 1475-2875