Table 2 Primers and conditions for the PROCR polymerase chain reaction (PCR) and ligase detection reactions (LDR)

PCR primers

Set 1

T(4414)C

C(4868)T

647 bp

Fw 5′-CCAGGTTATAATAAGCACTGAATCG-3′

Rv 5′-CTGGTACCACACGTGATAGGG-3′

95 °C 15 min

30 cycles:

95 °C 30 s

60 °C 30 s

72 °C 60 s

72 °C 10 min

Set 2

A(6936)G

Fw 5′-GGCCTATTCTTCGGGCTAAC-3′

251 bp

Rv 5′-TGGTGTTTCAGTTGGGGAGT-3′

LDR primers

4414 common

5′-PHOS-TGAGATCATGTCCTTTTCCTACTT-BIO-3′

95 °C 1 min

40 cycles:

95 °C 15 s

56 °C 2 min

4414 T(A)

5′-ctttctcatactttcaactaatttCTCCACCTGACCCAGGGA-3′

4414 C (G)

5′-ctttcttaatacattacaacatacCTCCACCTGACCCAGGGG-3′

4868 common

5′-PHOS-CTTCAGCCTGGGCCG-BIO-3′

4868 C (G)

5′-catcttcatatcaattctcttattCTGCGGGCAGAGTCAG-3′

4868 T (A)

5′-tactacttctataactcacttaaaCTGCGGGCAGAGTCAA-3′

6936 common

5′-PHOS-GTTTCATCATTGCTGGTGTG-BIO-3′

6936 A

5′-tacttaaacatacaaacttactcaCGTCCTGGTGGGCA-3′

6936 G

5′-atctcaattacaataacacacaaaCGTCCTGGTGGGCG-3′