Table 1 List of recombinant PfEMP1-VarO domains, sequence features and expression systems used to immunise mice
From: Functional analysis of monoclonal antibodies against the Plasmodium falciparum PfEMP1-VarO adhesin
VarO domain | Amino acid position in the complete protein sequence | Sequence features (number of potential N-glycosylation sites mutated S/T to A) | Expression system | Name used | References |
|---|---|---|---|---|---|
DBL1α1.6 | 1–487 | Recodoned (7) | Baculovirus/insect cells | bDBL1 | [13] |
E. coli/pMAL-c2X | eDBL1 | [27] | |||
CIDRγ6 | 399–835 | Recodoned (1) | Baculovirus/insect cells | bCIDR | [33] |
508–787 | Recodoned (1) | P. pastoris/pPICZαA | pCIDR | [20] | |
Head (DBL1α1.6–CIDRγ6) | 2–716 | Recodoned (8) | E. coli/pMAL-c2X | eHead | [20] |
DBL2β7 | 821–1242 | Recodoned (2) | Baculovirus/insect cells | bDBL2 | [33] |
DBL4ε5 | 1608–2014 | Recodoned (3) | E. coli/pMAL-c2X | eDBL4 | [20] |