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Table 2 ELISA titres, parasite immunoblot reactivity, surface reactivity and functional characteritics of anti-PfEMP1 VarO monoclonal IgGs

From: Functional analysis of monoclonal antibodies against the Plasmodium falciparum PfEMP1-VarO adhesin

mAb

Immunising antigen

Isotype

ELISA

Palo Alto 89F5 VarO iRBC

Immunoblot

Surface IFA

Rosette inhibitionb

Rosette disruptionb

SDS-extract

Total extract

50 % titrea

Endpoint titrea

Reduced

Non-reduced

Reduced

Non-reduced

Endpoint titrea

MFI max

100 %

100 %

50 %

D15-50

bDBL1

IgG1

10

0.01

Neg

Pos

Neg

Pos

1.6

700

1

100

5

D15-68

bDBL1

IgG1

10

0.03

Neg

Pos

Neg

Pos

10

400

>10

100

100

E20-76

bDBL1

IgG1

10

0.015

Neg

Pos

Neg

Pos

3.2

300

5

100

20

M21-17

bDBL1

IgG1

200

1.2

Neg

Neg

Neg

Pos

Neg

Neg

NA

NA

NA

M21-30

bDBL1

IgG1

330

1.2

Neg

Neg

Neg

Pos

Neg

Neg

NA

NA

NA

BDEE10

bDBL1

IgG1

10

0.03

ND

ND

Pos

Pos

16

200

>50

>100

40

BD20E4

bDBL1

IgG1

10

0.03

Neg

Pos

Neg

Pos

0.6

700

1

10

0.7

N6-37

pCIDR

IgG1

2

0.015

ND

ND

Neg

Pos

10

200

50

Nil

Nil

G8-49

eHead

IgG2b

20

0.1

ND

ND

Pos

Pos

2000

100

Nil

Nil

Nil

B12-15

bDBL2

IgG2a

1.4

0.005

Neg

Pos

Neg

Pos

3.2

400

Nil

Nil

Nil

B12-42

bDBL2

IgG2a

1.4

0.01

Neg

Pos

Neg

Pos

3.2

400

Nil

Nil

Nil

D18-94

eDBL4

IgG2a

15

0.01

ND

ND

Pos

Pos

10

350

Nil

Nil

Nil

Rabbitc

bDBL1

 

78

<4.8

Pos

Pos

Pos

Pos

5

650

25

>100

35

  1. ND not determined; NA not applicable; nil no inhibition or disruption at the highest concentration tested 50 and 100 µg ml−1 respectively
  2. aConcentration of monoclonal IgG (ng mL−1)
  3. bConcentration of mAb (µg mL−1)
  4. cRef [33]