From: Functional analysis of monoclonal antibodies against the Plasmodium falciparum PfEMP1-VarO adhesin
mAb | Immunising antigen | Isotype | ELISA | Palo Alto 89F5 VarO iRBC | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Immunoblot | Surface IFA | Rosette inhibitionb | Rosette disruptionb | ||||||||||
SDS-extract | Total extract | ||||||||||||
50 % titrea | Endpoint titrea | Reduced | Non-reduced | Reduced | Non-reduced | Endpoint titrea | MFI max | 100 % | 100 % | 50 % | |||
D15-50 | bDBL1 | IgG1 | 10 | 0.01 | Neg | Pos | Neg | Pos | 1.6 | 700 | 1 | 100 | 5 |
D15-68 | bDBL1 | IgG1 | 10 | 0.03 | Neg | Pos | Neg | Pos | 10 | 400 | >10 | ≫100 | 100 |
E20-76 | bDBL1 | IgG1 | 10 | 0.015 | Neg | Pos | Neg | Pos | 3.2 | 300 | 5 | ≫100 | 20 |
M21-17 | bDBL1 | IgG1 | 200 | 1.2 | Neg | Neg | Neg | Pos | Neg | Neg | NA | NA | NA |
M21-30 | bDBL1 | IgG1 | 330 | 1.2 | Neg | Neg | Neg | Pos | Neg | Neg | NA | NA | NA |
BDEE10 | bDBL1 | IgG1 | 10 | 0.03 | ND | ND | Pos | Pos | 16 | 200 | >50 | >100 | 40 |
BD20E4 | bDBL1 | IgG1 | 10 | 0.03 | Neg | Pos | Neg | Pos | 0.6 | 700 | 1 | 10 | 0.7 |
N6-37 | pCIDR | IgG1 | 2 | 0.015 | ND | ND | Neg | Pos | 10 | 200 | ≫50 | Nil | Nil |
G8-49 | eHead | IgG2b | 20 | 0.1 | ND | ND | Pos | Pos | 2000 | 100 | Nil | Nil | Nil |
B12-15 | bDBL2 | IgG2a | 1.4 | 0.005 | Neg | Pos | Neg | Pos | 3.2 | 400 | Nil | Nil | Nil |
B12-42 | bDBL2 | IgG2a | 1.4 | 0.01 | Neg | Pos | Neg | Pos | 3.2 | 400 | Nil | Nil | Nil |
D18-94 | eDBL4 | IgG2a | 15 | 0.01 | ND | ND | Pos | Pos | 10 | 350 | Nil | Nil | Nil |
Rabbitc | bDBL1 | 78 | <4.8 | Pos | Pos | Pos | Pos | 5 | 650 | 25 | >100 | 35 |