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Fig. 1 | Malaria Journal

Fig. 1

From: Plasmodium berghei bio-burden correlates with parasite lactate dehydrogenase: application to murine Plasmodium diagnostics

Fig. 1

a Qualitative detection of pLDH units in different blood components (undiluted) from a mouse infected with Plasmodium chabaudi AS. Individual bars represent negative and positive control from the kit, whole blood obtained from a naïve or P. chabaudi AS-infected BALB/c mouse, serum obtained from a naïve or P. chabaudi AS-infected BALB/c mouse or whole blood obtained from a P. chabaudi AS-infected mouse which had been drug treated. b Representation of a non-linear standard curve to quantify pLDH units (ng/mL). Recombinant pLDH from the kit was serially diluted in both PBS and blood and plotted as log 10 concentration of recombinant pLDH vs the OD. The dotted line shows the linear range of the curve. Each sample was tested in triplicate. Error bars represent the mean ± SE of experimental replicates. ****P value <0.0001, ns not significant. One-way ANOVA was used to determine the statistical significance between groups

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