Table 1 Mass spectrometer settings and MS parameters used for the detection of the test compounds on an API 3200 Q-Trap
Parameter | Compound 1 | Compound 2 |
|---|---|---|
Q1 mass (Da) | 531.2 | 490.2 |
Q3 mass (Da) | 91.1 | 261.2 |
Dwell time (ms) | 40 | 40 |
Declustering potential (V) | 91 | 61 |
Collision energy (V) | 89 | 73 |
Entrance potential (V) | 10 | 10 |
Collision cell exit potential (V) | 4 | 2 |
Source temperature (°C) | 500 | 500 |
Curtain gas (psi) | 25 | 25 |
Gas 1 (psi) | 50 | 50 |
Gas 2 (psi) | 70 | 70 |
CAD gas | Medium | Medium |
Ion spray voltage (kV) | 5500 | 5500 |
Ionization mode | Positive | Positive |