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Table 1 Limits of detection of Plasmodium falciparum under varied storage conditions of Plasmodium falciparum and Plasmodium vivax admixtures

From: An ultrasensitive reverse transcription polymerase chain reaction assay to detect asymptomatic low-density Plasmodium falciparum and Plasmodium vivax infections in small volume blood samples

  Sample collection storage conditions Admixture of P. falciparum and P. vivax
  3 days at 22 °C Frozen (−80 °C) 3 days at 28 °C 80 % RH 7 or 14 days at 28 °C 80 % RH Pf only Pv only Pf w/Pv plasmida Pv plasmid w/Pfb
Pf LoD (p/mL) 20.5 23.3 20.5 <16 21.3 25.1
Pv LoD (copies/μL) 19.7 49.5
  1. LoD Limits of detection; Pf P. falciparum; Pv P. vivax; RH relative humidity
  2. a3.8 × 104 plasmid copies/μL
  3. b1 × 104 p/mL P. falciparum