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Table 2 Sensitivity, specificity, positive predictive value and negative predictive value of the RT-PCR performed on simulated samples

From: An ultrasensitive reverse transcription polymerase chain reaction assay to detect asymptomatic low-density Plasmodium falciparum and Plasmodium vivax infections in small volume blood samples

Species test

P. falciparum

P. vivax

Positive

Negative

Positive

Negative

Species-specific RT-PCR

 Positive

522

0

154

0

 Negative

2

451

2

876

McNemar’s (two-tailed) test

P = 0.4795

Percentage

95 % CI

Percentage

95 % CI

 Sensitivity

100

99.30–100

100

97.66–100

 Specificity

99.56

98.41–99.95

99.77

99.18–99.97

 PPV

99.62

98.63–99.95

98.73

95.50–99.85

 NPV

100

99.19–100

100

99.58–100