Fig. 4
From: Upstream AUGs and upstream ORFs can regulate the downstream ORF in Plasmodium falciparum
Luciferase assay of the constructs containing var upstream region and upstream region with mutated ATGs. Firefly luciferase readings of the constructs Pf86, Pf86-60var and Pf86-60var(mut 1 to 6) obtained after transiently transfecting them in the parasite. Construct Pf86-stop was used as a negative control where the second codon of the luciferase CDS in the vector Pf86 was converted to a stop codon. Firefly luminescence units were normalized against those of Renilla for each construct. Normalized Firefly readings of each constructs have been expressed with respect to normalized Firefly readings of Pf86. Firefly luciferase readings and standard deviations have been calculated from five replicates. Normalized firefly readings of Pf86 were in the range of 1000–7000 RLUs, measured in a luminometer