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Fig. 7 | Malaria Journal

Fig. 7

From: Upstream AUGs and upstream ORFs can regulate the downstream ORF in Plasmodium falciparum

Fig. 7

Firefly luciferase readings of the plasmid Pf86 and its derivatives under different Kozak sequences with their frequencies. Firefly luciferase readings of the plasmid Pf86 and its derivatives carrying luciferase start codon under different Kozak sequences have been shown (bar graph). Frequency of occurrence of different Kozak sequences in the genome tested for their strength has been shown on secondary Y-axis (line). Firefly luminescence units were normalized against those of Renilla for each constructs. Normalized Firefly readings of each constructs have been expressed with respect to normalized Firefly readings of Pf86. Luciferase readings have been measured in a scintillation counter. Firefly luciferase readings and standard deviations have been calculated from four replicates. Normalized Firefly readings were in the range of ~800,000 to ~28,00,000 CPM, measured using a scintillation counter. Constructs containing different Kozak sequences were grouped into three categories based on the +4 position: those containing ‘G’ at +4 position (a), ‘C’ at +4 position (b) and ‘A’ at +4 position (c)

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