In vitro adaptation of Plasmodium falciparum reveal variations in cultivability

Malaria Journal

Table 1 Most favorable culture conditions and time to adaptation of Plasmodium falciparum parasites, ‘straight from the arm’ of 33 patient samples (eight conditions tested per patient)

Culture ID	Initial parasitaemia (%)	Culture 1		Culture 2
Culture ID	Initial parasitaemia (%)	Condition	Days to adapt	Condition	Days to adapt
CA1	0.2	2	9	6	Δ
CA2	1.1	2	10	5	10
CA3	1.9	2	11	1	18
CA4	2.6	2	11	3	11
CA5	2.7	2	13	5	13
CA6	13.5	2	16	NGT	–
CA7	0.7	2	18	8	18
CA8	3.8	8	20	7	Δ
CA9	0.0	2	22	1	Δ
CA10	0.5	3	21	5	Δ
CA11	3.6	4	12	1	23
CA12	6.0	5	13	8	Δ
CA13	2.6	7	22	5	Δ
CA14	1.9	6	9	6	Δ
CA15	0.1	6	15	1	Δ
CA16	3.3	5	16	6	19
CA17	9.7	6	18	8	Δ
CA18	0.9	6	20	1	22
CA19	1.3	6	20	2	Δ
CA20	9.2	6	23	8	Δ
CA21	0.4	7	33	NGT	–
NA1	0.4	NGT	–	NGT	–
NA2	0.2	NGT	–	NGT	–
NA3	0.4	NGT	–	NGT	–
NA4	0.6	NGT	–	NGT	–
NA5	0.0	NGT	–	NGT	–
NA6	0.0	NGT	–	NGT	–
NA7	3.6	NGT	–	NGT	–
NA8	2.0	NGT	–	NGT	–
NA9	0.5	2	Δ	NGT	–
NA10	1.6	NGT	–	NGT	–
NA11	2.0	6	Δ	NGT	–
NA12	1.7	5	Δ	7	Δ

Condition 1: Unwashed RBCs, HI-plasma, 1 % HCT
Condition 2: Unwashed RBCs, Albumax, 1 % HCT
Condition 3: Unwashed RBCs, HI-plasma, 0.25 % HCT
Condition 4: Unwashed RBCs, Albumax, 0.25 % HCT
Condition 5: Washed RBCS, HI-plasma, 1 % HCT
Condition 6: Washed RBCs, Albumax, 1 % HCT
Condition 7: Washed RBCs, HI-plasma, 0.25 % HCT
Condition 8: Washed RBCs, Albumax, 0.25 % HCT
Culture ID prefix CA- and NA- refer to ‘culture adapted’ and ‘non-culture adapted’, respectively
NGT refers to ‘no growth test’ was performed, as parasitaemia did not reach initiation milestones
Δ indicates that a culture did not pass growth tests during the 35 days of testing

ISSN: 1475-2875