|
Geographical setting
|
Gender
|
TOTAL
|
G6PD determination results
|
|---|
|
Biochemical test*
|
DNA analysis(**)
|
|---|
|
Normal(a)
|
G6PDd(#, b)
|
Wild-type allele
|
202G → A allele
|
376A → G allele
|
|---|
|
Cajigal municipality (Sucre state) n = 332
|
F
|
163
|
156 (95.7)
|
7 (4.3)
|
3
|
4
|
4
|
|
M
|
169
|
156 (92.3)
|
13 (7.7)
|
1
|
11
|
12
|
|
Sifontes municipality (Bolívar state) n = 332
|
F
|
164
|
163 (99.4)
|
1 (0.6)
|
1
|
0
|
0
|
|
M
|
168
|
165 (98.2)
|
3 (1.8)
|
0
|
2
|
3
|
| | |
664
|
640 (96.4)
|
24 (3.6)
|
5 (20.8)
|
17 (70.8)
|
19 (79.2)
|
- Data are in number (percentage) of cases for each condition
-
F females, M males, G6PDd G6PD-deficient subjects
-
#
p = 0.001 by Fisher’s exact test for the comparison of G6PD deficiency prevalence among subjects of Cajigal vs. Sifontes municipality
-
* The biochemical test used was a NeoLiSA G6PD assay (Interscientific®, Hollywood, USA). p < 0.0001 by Student’s test for comparison of mean G6PD enzyme activity among normal and G6PDd subjects
-
** DNA analysis by PCR/RFLP and sequencing of the exons 4–8 of G6PD gene from subjects with biochemical deficiency. The other alleles, which were studied by sequencing (185C → A, 542A → T, 592C → T, 593G → C, 634A → G, 637G → T, 680G → A, 820G → A, 835A → T, 854G → A and 871G → A) resulted wild-type
-
aMean G6PD enzyme activity of 12.9 ± 3.8 U/g Hb [defined as Class IV (in 84 %) and V (in 2 %) (data not shown)], according to WHO criteria (33, 34)
-
bMean G6PD enzyme activity of 4.5 ± 1.2 U/g Hb (defined as moderately deficient enzyme, Class III, according to WHO criteria [33, 34]. p = 0.007 among G6PDd males and females compared by Student’s test
