Skip to main content

Table 2 Prevalence of G6PD deficiency in Venezuelan individuals from Sifontes and Cajigal municipalities and G6PD gene alleles from subjects with biochemical deficiency

From: Prevalence and molecular characterization of G6PD deficiency in two Plasmodium vivax endemic areas in Venezuela: predominance of the African A-202A/376G variant

Geographical setting Gender TOTAL G6PD determination results
Biochemical test* DNA analysis(**)
Normal(a) G6PDd(#, b) Wild-type allele 202G → A allele 376A → G allele
Cajigal municipality (Sucre state) n = 332 F 163 156 (95.7) 7 (4.3) 3 4 4
M 169 156 (92.3) 13 (7.7) 1 11 12
Sifontes municipality (Bolívar state) n = 332 F 164 163 (99.4) 1 (0.6) 1 0 0
M 168 165 (98.2) 3 (1.8) 0 2 3
   664 640 (96.4) 24 (3.6) 5 (20.8) 17 (70.8) 19 (79.2)
  1. Data are in number (percentage) of cases for each condition
  2. F females, M males, G6PDd G6PD-deficient subjects
  3. # p = 0.001 by Fisher’s exact test for the comparison of G6PD deficiency prevalence among subjects of Cajigal vs. Sifontes municipality
  4. * The biochemical test used was a NeoLiSA G6PD assay (Interscientific®, Hollywood, USA). p < 0.0001 by Student’s test for comparison of mean G6PD enzyme activity among normal and G6PDd subjects
  5. ** DNA analysis by PCR/RFLP and sequencing of the exons 4–8 of G6PD gene from subjects with biochemical deficiency. The other alleles, which were studied by sequencing (185C → A, 542A → T, 592C → T, 593G → C, 634A → G, 637G → T, 680G → A, 820G → A, 835A → T, 854G → A and 871G → A) resulted wild-type
  6. aMean G6PD enzyme activity of 12.9 ± 3.8 U/g Hb [defined as Class IV (in 84 %) and V (in 2 %) (data not shown)], according to WHO criteria (33, 34)
  7. bMean G6PD enzyme activity of 4.5 ± 1.2 U/g Hb (defined as moderately deficient enzyme, Class III, according to WHO criteria [33, 34]. p = 0.007 among G6PDd males and females compared by Student’s test