Measurement of ex vivo ELISpot interferon-gamma recall responses to Plasmodium falciparum AMA1 and CSP in Ghanaian adults with natural exposure to malaria

Ganeshan, Harini; Kusi, Kwadwo A.; Anum, Dorothy; Hollingdale, Michael R.; Peters, Bjoern; Kim, Yohan; Tetteh, John K. A.; Ofori, Michael F.; Gyan, Ben A.; Koram, Kwadwo A.; Huang, Jun; Belmonte, Maria; Banania, Jo Glenna; Dodoo, Daniel; Villasante, Eileen; Sedegah, Martha

doi:10.1186/s12936-016-1098-8

Table 3 Activities to CSP peptide pools and CSP HLA peptide pools—matching activities of peptide pools

From: Measurement of ex vivo ELISpot interferon-gamma recall responses to Plasmodium falciparum AMA1 and CSP in Ghanaian adults with natural exposure to malaria

Subject	HLA supertype	CSP 15mer pool	CSP HLA pool	Interpretation
HLA-typed
v01	A01 A03 B27 B27	Cp9	C-HLA A03	*A03* Matched^a A03 pool contains promiscuous *A01* and *B27* epitopes^b
v02	A02 A03 B27 B44	NEG	C-HLA A01, C-HLA A02, C-HLA A03	*A02, A03* Matched^a A01 pool contains promiscuous *A03, B27* and *B44* epitopes^b A02 pool contains promiscuous *B27* epitope^b A03 pool contains promiscuous *B27* epitopes^b
v04	A01 A24 B27 B27	Cp1	NEG	Cp1 pool contains matched identified *A01, A24* and *B27* epitopes^c Cp1 pool contains matched promiscuous *A01, A24* and *B27* epitopes^d
v05	A03 A01A03 B27 B44	Cp1, Cp9	NEG	Cp1 pool contains matched identified *A03* and *B27* epitopes^c and promiscuous *A03* and *B44* epitopes^d Cp9 pool contains matched identified *B27* and promiscuous *B44* epitopes^d
v10	A02 A02 B07 B07	Cp9	NEG	Cp9 pool contains matched *A02* epitopes^c
v12	A01A24 A03 B07 B27	Cp6	NEG	Cp6 pool contains promiscuous *B07* and *B27* epitopes^d
v16	A03 A03 B27 B58	Cp9	C-HLA A01	A01 pool contains promiscuous *A03* and *B27* epitopes^b Cp9 pool contains promiscuous *A01, B27* and *B58* epitopes^d
HLA not typed
v13		NEG	C-HLA A24	Probably *A24* matched^b A24 pool contains promiscuous A02, B07, B27 and B58 epitopes^b
v14		Cp9	C-HLA A01, C-HLA A02, C-HLA A24	Probably *A02* matched as Cp9 contains identified A02 epitopes^a,c Cp9 pool contains promiscuous *A01* and *A24* epitopes^d
v30		NEG	C-HLA B27	Probably *B27* matched^a B27 pool contains identified promiscuous A01A24 and A02 epitopes, and predicted A01, A24, , B07, B58 and B62 epitopes^b
v23		Cp4	NEG	No HLA prediction possible^e
v31		Cp9	NEG	No HLA prediction possible^e
No. positive		9 (26%)	6 (17%)
Total Positive	12 (34%)

Activities of subjects to either CSP 15 mer peptide pools or CSP HLA peptide pools are shown; subjects that were negative with both sets of peptide pools were excluded. For each subject, the HLA supertypes that are matched by the tested peptides are italicized in bold and underlined, while predicted promiscuous epitopes are italicized and in bold. Positivity was defined as described in “Methods”
^aMatched, where activity of a CSP HLA pool matched that subject’s HLA
^bMatched through analysis of promiscuous epitopes in that positive CSP HLA pool and that match subject’s HLA
^cMatched through analysis of predicted epitopes in that positive CSP pool that match that subject’s HLA
^dMatched through analysis of promiscuous epitopes in that positive CSP pool that match that subject’s HLA
^eIn the absence of positive HLA peptide pools, no HLA prediction is possible

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ISSN: 1475-2875

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