Measurement of ex vivo ELISpot interferon-gamma recall responses to Plasmodium falciparum AMA1 and CSP in Ghanaian adults with natural exposure to malaria

Ganeshan, Harini; Kusi, Kwadwo A.; Anum, Dorothy; Hollingdale, Michael R.; Peters, Bjoern; Kim, Yohan; Tetteh, John K. A.; Ofori, Michael F.; Gyan, Ben A.; Koram, Kwadwo A.; Huang, Jun; Belmonte, Maria; Banania, Jo Glenna; Dodoo, Daniel; Villasante, Eileen; Sedegah, Martha

doi:10.1186/s12936-016-1098-8

Table 4 Activities to AMA1 peptide pools and AMA1 HLA peptide pools—matching activities of peptide pools

From: Measurement of ex vivo ELISpot interferon-gamma recall responses to Plasmodium falciparum AMA1 and CSP in Ghanaian adults with natural exposure to malaria

Subject	HLA supertype	AMA1 15mer pool	AMA1 HLA pool	Interpretation
HLA-typed
v01	A01 A03 B27 B27	Ap1, Ap6	A01, B44	*A01* Matched^a B44 pool contains promiscuous *A01* and *B27* epitopes^b A01 pool contains promiscuous *A03* and *B27* epitopes^b Ap1 and Ap6 pools contain promiscuous *A01, A03* and *B27* epitopes^d
v02	A02 A03 B27 B44	NEG	A02	*A02* Matched^a A02 contains promiscuous *A01, A03* and *B44* epitopes^d
v03	A01 A03 B07 B58	NEG	A02	A02 pool contains promiscuous *A01* and *A03* epitopes^b
v04	A01 A24 B27 B27	Ap2, Ap6	NEG	Ap2 pool contains matching *A01* epitopes^c Ap6 pool contains matching *A01* epitopes^c, and promiscuous *B27* epitopes^d
v05	A03 A01A03 B27 B44	Ap6	A03	*A03* Matched^a A03 contains promiscuous *A01A03, A03* and *B27* epitopes^d
v08	A01A03 A02 B27 B44	Ap1	NEG	Ap1 pool contains matching *A02* and *B44* epitopes^c, and promiscuous *B27* epitopes^d
v10	A02 A02 B07 B07	NEG	A02, A03	*A02* matched^a A03 pool contains promiscuous *A02* and *B07* epitopes^d
v12	A01A24 A03 B07 B27	NEG	A02	A02 pool contains promiscuous *A03* and *B27* epitopes^d
v16	A03 A03 B27 B58	Ap2, Ap6, Ap11	A02	A02 contains promiscuous *A03* and *B27* epitopes^d Ap6 contains promiscuous *A03, B27* and *B58* epitopes^d
v17	A01A03 A03 B07 B44	Ap6	NEG	Ap6 contains matching *A03* and *B07* epitopes^c, and promiscuous *A01A03* and *B07* epitopes^d
v19	A03 A24 B44 B62	NEG	A03	*A03* Matched^a
HLA not typed
v06		Ap1	A02, A03	Ap1 pool contains matching *A02* and *A03* epitopes^e
v09		Ap1, Ap6	B07	Ap1 and Ap6 pools contain matching *B07* epitopes^e
v13		Ap7	A24	Ap7 and A24 contains promiscuous *A01, A02* and *B44* epitopes^e,f
v14		Ap1, Ap2, Ap6	A01, A02, A03, A24, B07	Ap1 pool contains matching *A01, A02, A03, A24, B07* and *B44* epitopes^e Ap2 contains matching *A01, A02* and *A03* epitopes^e, and promiscuous *B07* epitopes^f Ap6 contains matching *A01, A03* and *B07* epitopes⁵, and promiscuous *B07* epitopes^f
v22		Ap6	A03	Ap6 pool contains matching *A03* epitopes^e
v30		Ap1, Ap2, Ap3, Ap6	A03, B07	Ap1, Ap2, Ap3 and Ap6 pools contain matching *A03* epitopes^e Ap1 and Ap6 pools contain matching *B07* epitopes^e
v31		Ap6	A03	Ap6 pool contains matching *A03* epitopes^e
v32		Ap1	A03	Ap1 pool contains matching *A03* epitopes^e
v33		Ap1, Ap6	A03	Ap1 and Ap6 pools contain matching *A03* epitopes^e
v18		Ap3, Ap4, Ap7, Ap8, Ap10	NEG	No prediction possible^g
v07		Ap1, Ap6, Ap11	NEG	No prediction possible^g
v20		NEG	A03	Probably contains *A03*-matched epitopes^e
v21		Ap2	NEG	No prediction possible^g
v23		Ap6	NEG	No prediction possible^g
v26		Ap6	NEG	No prediction possible^g
v28		Ap6	NEG	No prediction possible^g
v34		Ap6	NEG	No prediction possible^g
No. positive		22 (63%)	18 (51%)
Total Positive	28 (80%)

Activities of subjects to either AMA1 15 mer peptide pools or AMA1 HLA peptide pools are shown; subjects that were negative with both sets of peptide pools were excluded. For each subject, the HLA supertypes that are matched by the tested peptides are italicized in bold and underlined, while predicted promiscuous epitopes are italicized and in bold. Positivity was defined as described in “Methods”
Interpretation (for HLA-typed subjects)
^aMatched, where activity of an AMA1 HLA pool matched that subject’s HLA
^bMatched through analysis of promiscuous epitopes in that positive AMA1 HLA pool and that subject’s HLA
^cHLA-Matched through analysis of epitopes in that positive AMA1 pool that match that subject’s HLA
^dHLA-Matched through analysis of promiscuous epitopes in that positive AMA1 pool that match that subject’s HLA. For non-HLA-typed subjects
^eHLA-matched through analysis of epitopes in that positive AMA1 peptide pool that contain matched HLA epitopes
^fHLA-matched through analysis of promiscuous epitopes in that positive AMA1 peptide pool that contain matched HLA epitopes
^gIn the absence of positive HLA peptide pools, no HLA prediction is possible

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ISSN: 1475-2875

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