Fig. 2

VWF multimer distribution. The VWF multimer distribution was examined in baseline serum samples from either SM patients or pooled normal plasma (PNP) by electrophoresis on a non-reducing 1.7 % agarose-SDS gel, followed by western blotting. The Y axis shows the approximate number of VWF dimers at each level of the gel. Patients 2, 4, 5, 8–10, and 12 had larger VWF multimers than those found in normal plasma, with two to four larger bands (on top of the multimers) detected by densitometry analysis using ImageQuant TL software (GE Healthcare Bio-Sciences, Pittsburgh, PA, USA)