Skip to main content
Fig. 3 | Malaria Journal

Fig. 3

From: Mutations of pvdhfr and pvdhps genes in vivax endemic-malaria areas in Kota Marudu and Kalabakan, Sabah

Fig. 3

PCR–RFLP of the pvdhfr gene. PCR–RFLP product of VDNF 57/VDF NR with BsrGI showed no cleavage of the 472 bp indicating wild type Phe58 and mutation 57Leu (a). Reaction with PvuII showed no cleavage (472 bp) indicating mutation 117Thr/Asn (b). Reaction with BstN1 cleaved the 472 bp to 258 and 215 bp indicating mutation 117Thr (c). Reaction with Bsr1 showed no cleavage indicating wild type Ser118 and mutation 117Thr (d). a, b, c and d: Lane L DNA ladder of Agilent DNA 1000 Kit (Agilent Technologies, Molecular Probes Inc, USA), Lanes 1–12 PCR–RFLP products of samples

Back to article page