Fig. 4

PCR–RFLP of the pvdhfr gene. PCR–RFLP of VDTOF/VDFNR with StyI cleaved the 608 bp fragment into 472 and 136 bp indicating of wild type Ile173 (a). Reaction with XmnI showed no cleavage (608 bp) indicating mutation 57Ile/Leu (b). a and b: Lane L DNA ladder of Agilent DNA 1000 Kit (Agilent Technologies, Molecular Probes Inc, USA). Lanes 1–12 PCR-RFLP products of samples