Table 1 Primers and profiles used for amplification of pvdhfr gene
Primer pairs (5′–3′) | Concentration of Mg2 (mM) | Concentration of primer (nM) | Annealing Temperature (°C/Min) | Cycle | Size (bp) |
|---|---|---|---|---|---|
VDT OF: ATGGAGGACCTTTCAGATGTATTTGACATT VDT OR: GGCGGCCATCTCCATGGTTATTTTATCGTG | 2.0 | 200 | 68/2 | 25 | 1876 |
VDT OF: ATGGAGGACCTTTCAGATGTATTTGACATT VDF OR: CTTGCTGTAAACCAAAAAGTCCA | 2.0 | 400 | 66/2 | 30 | 711 |
VDF 13NF: GACCTTTCAGATGTATTTGACATTTACGGC VDF NR58: GGTACCTCTCCCTCTTCCACTTTAGCTTCT | 1.5 | 300 | 62/2 | 30 | 232 |
VDNF57: CATGGAAATGCAACTCCGTCGATATGATGT VDF NR: TCACACGGGTAGGCGCCGTTGATCCTCGTG | 1.5 | 400 | 50/2 | 30 | 472 |
VDT OF: ATGGAGGACCTTTCAGATGTATTTGACATT VDF NR: TCACACGGGTAGGCGCCGTTGATCCTCGTG | 1.0 | 300 | 60/2 | 30 | 608 |