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Table 3 The primer pairs, PCR product sizes, restriction enzymes used and the digestion product size in detection of gene polymorphism on pvdhfr and pvdhps gene

From: Mutations of pvdhfr and pvdhps genes in vivax endemic-malaria areas in Kota Marudu and Kalabakan, Sabah

Gene/primer pair PCR product size (bp) To detectmutation at codon Restriction enzyme used Digestion product size (bp)
Wild type Mutation
DHFR
Forward: VDF 13NF
Reserve: VDF NR58
232 Ile13Leu
Pro33Leu
Ser58Arg
Thr61Met
HaeII
SacII
AluI
Tsp451
Ile: 232
Pro: 138 and 94
Ser: 167, 40 and 25
Thr: 200 and 32
Leu: 200 and 32
Leu: 232
Arg: 207 and 25
Met: 232
DHFR/
Forward: VDNF57
Reserve: VDF NR
472 Phe57Ile/Leu BsrGI
PvuII
BstNI
BsrI
Phe: 472
Ser: 258 and 214
Ser: 472
Ser: 472
Ile: 444 and 28
Leu: 472
Asn/Thr: 472
Thr: 257 and 215
Asn: 472
Asn: 253 and 219
Thr: 472
DHFR/
Forward: VDT OF
Reverse: VDF NR
608 Phe57Ile/Leu
Ile173Leu
XmnI
StyI
Phe: 442 and 166
Ile: 472 and 136
Ile/Leu: 608
Leu: 438, 97 and 73
DHPS/
Forward: VDHPS NF
Reverse: VDHPS NR
703 Ala383Gly MspI Ala: 703 Gly: 655 and 48
DHPS/
Forward: VDHPS553OF
Reverse: VDHPSNR
170 Ala553Gly MscI Ala: 143 and 27 Gly: 170