Genetic diversity and natural selection in the rhoptry-associated protein 1 (RAP-1) of recent Plasmodium knowlesi clinical isolates from Malaysia

Table 1 Estimates of DNA diversity, selection, and neutrality tests of PkRAP-1 in Malaysia

PkRAP-1	n	S	Hd ± SD	π ± SD	d_N ± SE	d_S ± SE	d_N/d_S	Z test	Tajima’s D	Fu and Li’s D
Exon 1	34	276	0.818 ± 0.054	0.00915 ± 0.00089	0.00574 ± 0.00352	0.02253 ± 0.01104	0.25477	d_N = d_S	−0.44307 (P > 0.10)	−0.47531 (P > 0.10)
Exon 2	34	1929	0.993 ± 0.009	0.01353 ± 0.00102	0.00894 ± 0.00145	0.03274 ± 0.00591	0.27306	d_N < d_S (P < 0.05)	−0.20877 (P > 0.10)	−0.22130 (P > 0.10)
Total CDS	34	2205	0.995 ± 0.009	0.01298 ± 0.00091	0.00854 ± 0.00126	0.03137 ± 0.00483	0.27223	d_N < d_S (P < 0.05)	−0.23957 (P > 0.10)	−0.26919 (P > 0.10)

n number of sequences, S number of sites, Hd haplotype diversity, π observed average pairwise nucleotide diversity, d _N rate of non-synonymous substitutions per non-synonymous site, d _S rate of synonymous substitutions per synonymous site

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